ABSTRACT
The effect of Sepia on the activity of tyrosine protein kinase (TPK) protein kinase C (PKC) and cAMP-dependent protein kinase (PKA) was studied in H 22 cancer cells by using the incorporation of ? 32 P ATP into exogenous substract.The results showed that membranous TPK activity of membrane,cytosol and mitochondria was elevated significantly,while the membranous TPK activity was increased obviously;while the cytosolic PKC activity was increased most.The cytosolic PKA activity was increased.These results suggested that Sepia can changeover the activity of TPK,PKC and PKA,which was abnormally changed by cancer cells,then caused that the up regulation was diminished and the down regulation was enhanced towards the Ras MAPK signal transduction pathways.This inhibited or interrupted the cascade transduction of the Ras MAPK pathway,thus produced the effect of anticancer and accelerated the differentiation by transforming cancer cells from undifferentiated proliferation to differentiated proliferation.
ABSTRACT
Objective To study the antitumor mechanism of DHA compound. Methods RT-PCR was used to investigate the effects of DHA compound on H-Ras and pl6 mRNA transcription in gastric adenocarcinoma. Results Compared with control, the content of H-Ras mRNA was decreased and the content of p16 mRNA was increased in gastric adenocarcinoma significantly after treated with DHA compound. Conclusion The transcription of H-Ras gene was decreased and the transcription of pl6 gene was increased in gastric adenocarcinoma after treated with DHA compound and thus the cancer cell growth was inhibited.